Publication

TIA-1 Self-Multimerization, Phase Separtion, and Recruitment into Stress Granules Are Dynamically Regulated by Zn2+

Citation:
Cell Reports. 2018 Jan 2;22(1):59-71. doi: 10.1016/j.celrep.2017.12.036. PMID: 29298433.
Authored By:
Rayman JB, Karl KA, Kandel ER.
Abstract:
Stress granules are non-membranous structures that transiently form in the cytoplasm during cellular stress, where they promote translational repression of non-essential RNAs and modulate cell signaling by sequestering key signal transduction proteins. These and other functions of stress granules facilitate an adaptive cellular response to environmental adversity. A key component of stress granules is the prion-related RNA-binding protein, T cell intracellular antigen-1 (TIA-1). Here, we report that recombinant TIA-1 undergoes rapid multimerization and phase separation in the presence of divalent zinc, which can be reversed by the zinc chelator, TPEN. Similarly, the formation and maintenance of TIA-1-positive stress granules in arsenite-treated cells are inhibited by TPEN. In addition, Zn2+ is released in cells treated with arsenite, before stress granule formation. These findings suggest that Zn2+ is a physiological ligand of TIA-1, acting as a stress-inducible second messenger to promote multimerization of TIA-1 and subsequent localization into stress granules.
Published in:
Cell Reports

More Publications

August 1, 2023

Journal of Psychopharmacology

Improving Translational Relevance in Preclinical Psychopharmacology (iTRIPP)

July 6, 2023

Gerontology and Geriatric Medicine

Caring for Dementia Caregivers: Understanding Caregiver Stress During the COVID-19 Pandemic

May 19, 2023

Nature Reviews Neurology

Global synergistic actions to improve brain health for human development

April 20, 2023

Nature Mental Health

Machine learning-based identification of a psychotherapy-predictive electroencephalographic signature in PTSD

February 21, 2023

Translational Psychiatry

Screening for PTSD and TBI in Veterans using Routine Clinical Laboratory Blood Tests

February 13, 2023

Frontiers in Aging Neuroscience

Machine learning within the Parkinson’s progression markers initiative: Review of the current state of affairs